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This is caused by a connection error between your computer and the Arduino, and can result from many different specific problems. Here are some things that can fix this error:

  • Disconnect and reconnect the USB cable.
  • Use a different USB cable.
  • Press the reset button on the board.
  • Make sure you select the right board in Tools ► Board ►, e.g. If you are using the Duemilanove 328, select that instead of Duemilanove 128. The board should say what version it is on the microchip.
  • Make sure you selected the right port in Tools ► Serial Port ►. In extreme cases, you may need to burn the bootloader. There is a good guide on how to do that here: http://arduino.cc/en/Hacking/Bootloader?from=Main.Bootloader.
  • Restart the Arduino IDE.