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Identification of genes that may regulate the expression of the transcription factor production of anthocyanin pigment 1 (PAP1)/MYB75 involved in Arabidopsis anthocyanin biosynthesis

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Abstract

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A putative RNA-binding protein with a single RNA Recognition Motif (At3G63450) is involved in anthocyanin biosynthesis via its ability to modulate the transcript level of a major positive regulator PAP1 in Arabidopsis.

Abstract

The R2R3 MYB-activator production of anthocyanin pigment 1 (PAP1)/MYB75 plays a major role in anthocyanin biosynthesis in Arabidopsis in combination with one of three bHLH activators including transparent test 8 (TT8), enhancer of glabra3 (EGL3), glabra3 (GL3), and the WD-repeat transcription factor transparent testa 1 (TTG1), forming ternary MYB-basic HLH-WD40 complexes. Transcriptional activation of PAP1 expression is largely triggered by changes in light color and intensity, temperature fluctuations, nutrient status, and sugar and hormone treatments. However, the immediate upstream and downstream regulatory factors for PAP1 transcription are largely unknown. In the present study, using a T-DNA insertional mutagenesis approach, we transformed pap1-Dominant (pap1D) plants to modulate the levels of endogenous PAP1 transcripts. We employed Restriction Site Extension (RSE)-PCR analysis of 247 homogenous T3 genetic mutant lines exhibiting variations in anthocyanin accumulation compared to pap1D and identified 92 lines with T-DNA integrated in either intra- or inter-genic locations. This analysis revealed 80 novel candidate proteins, including a putative RNA-binding protein with a single RNA Recognition Motif (At3G63450), which may directly or indirectly regulate PAP1 expression at the transcriptional level.

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Acknowledgments

This work was supported by Grant PJ8205 from the Next-Generation BioGreen 21 Program, Rural Development Administration, and Grant 2011-0031343 from the Advanced Biomass Research and Development Center, Republic of Korea.

Conflict of interest

The authors have no competing financial interests to declare.

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Author notes

  1. Misuk Cho

    Present address: Department of Eco-Biological Science, Woosuk University, Jincheon-eup, Jincheon-gun, Chungcheongbuk-do, 365-800, South Korea

Authors and Affiliations

  1. Department of Biological Sciences, Chungnam National University, 99 Daehagro, Youseong, Daejeon, 305-764, Korea

    Dong Ho Shin, Misuk Cho, Myoung Goo Choi, Seul-Ki Lee & Youn-Il Park

  2. DBT-AAU Centre for Agricultural Biotechnology, Assam Agricultural University, Jorhat, India

    Prasanta Kumar Das

  3. Division of Biosciences and Bioinformatics, Myongji University, Yongin, 449-728, Korea

    Sang-Bong Choi

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  1. Dong Ho Shin
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Correspondence to Youn-Il Park.

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Communicated by J. S. Shin.

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Shin, D.H., Cho, M., Choi, M.G. et al. Identification of genes that may regulate the expression of the transcription factor production of anthocyanin pigment 1 (PAP1)/MYB75 involved in Arabidopsis anthocyanin biosynthesis. Plant Cell Rep 34, 805–815 (2015). https://doi.org/10.1007/s00299-015-1743-7

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