Abstract
In the last decades, pulsed electric fields (PEF) have been proposed as alternative or complementary to traditional food processing technologies in order to improve the competitiveness of the food industry. PEF has been suggested as a technology of choice to obtain safe and high-quality plant-based foods with a shelf-life similar to the attained with mild heat pasteurization treatments. On the other hand, the application of PEF as a pretreatment for the permeabilization of vegetable tissues has been demonstrated to enhance the efficiency of mass transfer of water or of valuable compounds from biological matrices in drying, extraction, and diffusion processes. Moreover, PEF treatments are currently under study to prospect their potential to induce stress reactions in plant systems, so that bioproduction of certain compounds can be enhanced or stimulated. However, the impact of different PEF processing strategies on health-related compounds of plan-based foods has not been always considered. These review aims to present recent results regarding the effects of PEF on health-related properties of plant-based foods, including those preserved by PEF and those obtained from PEF-assisted and PEF-stressed processing.

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References
Aadil RM, Zeng XA, Ali A, Zheng F, Farrooq MA, Han Z, Khalid S, Jabbar S (2015) Influence of different pulsed electric field strengths on the quality of the grapefruit juice. Int J Food Sci Technol 50:2290–2296
Abenoza M, Benito M, Saldaña G, Álvarez I, Raso J, Sánchez-Gimeno AC (2013) Effects of pulsed electric field on yield extraction and quality of olive oil. Food Bioprocess Technol 6:1367–1373
Ade-Omowaye BIO, Angersbach A, Taiwo KA, Knorr D (2001) The use of pulsed electric fields in producing juice from paprika (Capsicum annuum L.) J Food Process Preserv 25:353–365
Ade-Omowaye BIO, Rastogi NK, Angersbach A, Knorr D (2002) Osmotic dehydration of bell peppers: influence of high intensity electric field pulses and elevated temperature treatment. J Food Eng 54:35–43
Ade-Omowaye BIO, Taiwo KA, Eshtiaghi NM, Angersbach A, Knorr D (2003) Comparative evaluation of the effects of pulsed electric field and freezing on cell membrane permeabilisation and mass transfer during dehydration of red bell peppers. Innov Food Sci Emerg 4:177–188
Agcam E, Akyildiz A, Evrendilek GA (2014) Comparison of phenolic compounds of orange juice processed by pulsed electric fields (PEF) and conventional thermal pasteurisation. Food Chem 143:354–361
Aguilar-Rosas SF, Ballinas-Casarrubias ML, Nevarez-Moorillon GV, Martin-Belloso O, Ortega-Rivas E (2007) Thermal and pulsed electric fields pasteurization of apple juice: Effects on physicochemical properties and flavour compounds. Journal of Food Engineering 83(1):41–46
Barba FJ, Jäger H, Meneses N, Esteve MJ, Frígola A, Knorr D (2012) Evaluation of quality changes of blueberry juice during refrigerated storage after high-pressure and pulsed electric fields processing. Innov Food Sci Emerg Technol 14:18–24
Barba FJ, Parniakov O, Pereira SA, Wiktor A, Grimi N, Boussetta N, Saraiva JA, Raso J, Martin-Belloso O, Witrowa-Rajchert D, Lebovka N, Vorobiev E (2015) Current applications and new opportunities for the use of pulsed electric fields in food science and industry. Food Res Int 77:773–798
Bellostas N, Sørensen AD, Sørensen JC, Sørensen H (2007) Genetic variation and metabolism of glucosinolates. Adv Bot Res 45:369–415
Bobinaite R, Pataro G, Lamanauskas N, Satkauskas S, Viskelis P, Ferrari G (2015) Application of pulsed electric field in the production of juice and extraction of bioactive compounds from blueberry fruits and their by-products. J Food Sci Technol 52:5898–5905
Cai Z, Riedel H, Min N, Kütük O, Mewis I, Jäger H, Knorr D, Smetanska I (2011) Effects of pulsed electric field on secondary metabolism of Vitis vinifera L. cv. Gamay Fréaux suspension culture and exudates. Appl Biochem Biotechnol 164:443–453
Carbonell JM, Buniowska M, Braba FJ, Grimi N, Vorobiev E, Esteve MJ, Frígola A (2016) Changes of antioxidant compounds in a fruit juice-Stevia rebaudiana blend processed by pulsed electric technologies and ultrasound. Food Bioprocess Technol 9:1159–1168
Cortés C, Esteve MJ, Rodrigo D, Torregrosa F, Frígola A (2006a) Changes of color and carotenoids contents during high-intensity pulsed electric field treatment in orange juices. Food Chem Toxicol 44:1932–1939
Cortés C, Torregrosa F, Esteve MJ, Frígola A (2006b) Carotenoid profile modification during refrigerated storage in untreated and pasteurized orange juice and orange juice treated with high-intensity pulsed electric fields. J Agric Food Chem 54:6247–6254
Day J, Mumper RJ (2010) Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules 15:7313–7352
Donsi F, Ferrari G, Pataro G (2010) Applications of pulsed electric field treatments for the enhancement of mass transfer from vegetable tissue. Food Eng Rev 2:109–130
Elez-Martínez P, Soliva-Fortuny R, Martín-Belloso O (2006) Comparative study on shelf-life of orange juice processed by high intensity pulsed electric fields or heat treatment. Eur Food Res Technol 222:321–329
Elez-Martínez P, Martín-Belloso O (2007) Effects of high intensity pulsed electric field processing conditions on vitamin C and antioxidant capacity of orange juice and gazpacho, a cold vegetable soup. Food Chem 102:201–209
Elez-Martínez P, Soliva-Fortuny R, Martín-Belloso O (2009) Impact of high-intensity pulsed electric fields on bioactive compounds in Mediterranean plant-based foods. Nat Prod Commun 4:741–746
Frandsen HB, Markedal KE, Martín-Belloso O, Sánchez-Vega R, Soliva-Fortuny R, Sørensen H, Sørensen S, Sørensen JC (2014) Effects of novel processing techniques on glucosinoales and membrane associated myrosinases in broccoli. Polish J Food Nutr Sci 64:17–25
Galindo F, Dejmek P, Lundgren K, Rasmusson A, Vicente A, Moritz T (2009) Metabolomic evaluation of pulsed electric field-induced stress on potato tissue. Planta 230:469–479
Garde-Cerdán T, Arias-Gil M, Marsellés-Fontanet R, Ancín-Azpilicueta C, Martín-Belloso O (2007) Effects of thermal and non-thermal processing treatments on fatty acids and free amino acids of grape juice. Food Control 18:473–479
Gómez Galindo F, Wadso L, Vicente A, Dejmek P (2008) Exploring metabolic responses of potato tissue induced by electric pulses. Food Biophys 3:352–360
Grimi N, Lebovka N, Vorobiev E, Vaxelaire J (2009) Effect of a pulsed electric field treatment on expression behavior and juice quality of Chardonnay grape. Food Biophys 4:191–198
Grimi N, Mamouni F, Lebovka N, Vorobiev E, Vaxelaire J (2011) Impact of apple processing modes on extracted juice quality: pressing assisted by pulsed electric fields. J Food Eng 103:52–61
Grymonpré DR, Sharma AK, Finney WC, Locke BR (2001) The role of Fenton’s reaction in aqueous phase pulsed streamer corona reactors. Chem Eng J 82:189–207
Guderjan M, Töpfl S, Angersbach A, Knorr D (2005) Impact of pulsed electric field treatment on the recovery and quality of plant oils. J Food Eng 67:281–287
Guderjan M, Elez-Martínez P, Knorr D (2007) Application of pulsed electric fields at oil yield and content of functional food ingredients at the production of rapeseed oil. Innov Food Sci Emerg 8:55–62
Gueven A, Knorr D (2011) Isoflavonoid production by soy plant callus suspension culture. J Food Eng 103(3):237–243
Gürsul I, Gueven A, Grohmann A, Knorr D (2016) Pulsed electric fields on phenylalanine ammonia lyase activity of tomato cell cultures. J Food Eng 188:66–76
Halkier BA, Gershenzon J (2006) Biology and biochemistry of glucosinolates. Plant Biol 57:303–333
Jaeger H, Schulz M, Lu P, Knorr D (2012) Adjustment of milling, mash electroporation and pressing for the development of a PEF assisted juice production in industrial scale. Innov Food Sci Emerg 14:46–60
Jeffery EH, Araya M (2009) Physiological effects of broccoli consumption. Phytochem Rev 8:283–298
Lamanauskas N, Satkauskas S, Bobinaite R, Viskelis P (2015) Pulsed electric field (PEF) impact on Actinidia kolomikta drying efficiency. J Food Process Eng 38:243–249
Leong SY, Burritt DJ, Oey I (2016) Evaluation of the anthocyanin release and health promoting properties of Pinot Noir grape juices after pulsed electric fields. Food Chem 196:833–841
Lamanauskas N, Pataro G, Bobinas C, Satkauskas S, Viskelis P, Bobinaite R, Ferrari G (2016) Impact of pulsed electric field treatment on juice yield and recovery of bioactive compounds from raspberries and their by-products. Zemdirbyste-Agriculture 103:83–90
López-Alfaro I, González-Arenzana L, López N, Santamaría P, López R, Garde-Cerdán T (2013) Pulsed electric field treatment enhanced stilbene content in Graciano, Tempranillo and Grenache grape varieties. Food Chem 141:3759–3765
Marsellés-Fontanet AR, Puig-Pujol A, Olmos P, Mínguez-Sanz S, Martín-Belloso O (2013) A comparison of the effects of pulsed electric field and thermal treatments on grape juice. Food Bioprocess Technol 6:978–987
Morales-de la Peña M, Salvia-Trujillo L, Rojas-Graü MA, Martín-Belloso O (2010) Impact of high intensity pulsed electric field on antioxidant properties and quality parameters of fruit juice-soymilk beverage in chilled storage. LWT-Food Sci Technol 43:872–881
Morales-de la Peña M, Salvia-Trujillo L, Rojas-Graü MA, Martín-Belloso O (2011a) Changes on phenolic and carotenoid composition of high intensity pulsed electric field and thermally treated fruit juice-soymilk beverages during refrigerated storage. Food Chem 129:982–990
Morales de la Peña M, Salvia-Trujillo L, Rojas-Graü MA, Martín-Belloso O (2011b) Impact of high intensity pulsed electric fields or heat treatments on the fatty acid and mineral profiles of a fruit juice-soymilk beverage during storage. Food Control 22:1975–1983
Morales-de la Peña M, Salvia-Trujillo L, Rojas-Graü MA, Martín-Belloso O (2016a) Isoflavone profile of a high intensity pulsed electric field or thermally treated fruit juice-soymilk beverage stored under refrigeration. Innov Food Sci Emerg Technol 11(4):604–610
Morales-de la Peña M, Salvia-Trujillo L, Rojas-Graü MA, Martín-Belloso O (2016b) Effects of high intensity pulsed electric fields or thermal pasteurization and refrigerated storage on antioxidant compounds of fruit juice-milk beverages. Part I: Phenolic acids and flavonoids. J. Food Process Preserv. doi:10.1111/jfpp.12912.
Noci F, Riener J, Walkling-Ribeiro M, Cronin DA, Morgan DJ, Lyng JG (2008) Ultraviolet irradiation and pulsed electric fields (PEF) in a hurdle strategy for the preservation of fresh apple juice. J Food Eng 85(1):141–146
Odriozola-Serrano I, Bendicho-Porta S, Martín-Belloso O (2006) Comparative study of shelf-life of whole milk processed by high-intensity pulsed electric field or heat treatment. J Dairy Sci 89:905–911
Odriozola-Serrano I, Aguiló-Aguayo I, Soliva-Fortuny R, Gimeno-Añó V, Martín-Belloso O (2007) Lycopene, vitamin C, and antioxidant capacity of tomato juice as affected by high-intensity pulsed electric fields critical parameters. J Agric Food Chem 55:9036–9042
Odriozola-Serrano I, Soliva-Fortuny R, Martín-Belloso O (2008b) Changes of health-related compounds throughout cold storage of tomato juice stabilized by thermal or high intensity pulsed electric field treatments. Innov Food Sci Emerg Technol 9:272–279
Odriozola-Serrano I, Soliva-Fortuny R, Martín-Belloso O (2008d) Phenolic acids, flavonoids, vitamin C and antioxidant capacity of strawberry juices processed by high-intensity pulsed electric fields or heat treatments. Eur Food Res Technol 228:239–248
Odriozola-Serrano I, Soliva-Fortuny R, Gimeno-Añó V, Martín-Belloso O (2008a) Modeling changes in health-related compounds of tomato juice treated by high-intensity pulsed electric fields. J Food Eng 89:210–216
Odriozola-Serrano I, Soliva-Fortuny R, Gimeno-Añó V, Martín-Belloso O (2008c) Kinetic study of anthocyanins, vitamin C, and antioxidant capacity in strawberry juices treated by high-intensity pulsed electric fields. J Agric Food Chem 56:8387–8393
Odriozola-Serrano I, Soliva-Fortuny R, Martín-Belloso O (2009a) Impact of high-intensity pulsed electric fields variables on vitamin C, anthocyanins and antioxidant capacity of strawberry juice. LWT-Food Sci Technol 42:93–100
Odriozola-Serrano I, Soliva-Fortuny R, Hernández-Jover T, Martín-Belloso O (2009b) Carotenoid and phenolic profile of tomato juice processed by high intensity pulsed electric fields compared to conventional thermal treatments. Food Chem 112:258–266
Odriozola-Serrano I, Aguiló-Aguayo I, Soliva-Fortuny R, Martín-Belloso O (2013) Pulsed electric fields processing effects on quality and health-related constituents of plant-based foods. Trends Food Sci Tech 29:98–107
Oms-Oliu G, Odriozola-Serrano I, Soliva-Fortuny R, Martín-Belloso O (2009) Effects of high-intensity pulsed electric field processing conditions on lycopene, vitamin C and antioxidant capacity of watermelon juice. Food Chem 115:1312–1319
Plaza L, Sánchez-Moreno C, De Ancos B, Elez-Martínez P, Martín-Belloso O, Cano MP (2011) Carotenoid and flavanone content during refrigerated storage of orange juice processed by high-pressure, pulsed electric fields and low pasteurization. LWT-Food Sci Technol 44:834–839
Puértolas E, Martínez de Marañón I (2015) Olive oil pilot-production assisted by pulsed electric field: impact on extraction yield, chemical parameters and sensory properties. Food Chem 167:497–502
Quitão-Teixeira LJ, Odriozola-Serrano I, Soliva-Fortuny R, Mota-Ramos A, Martín-Belloso O (2009) Comparative study on antioxidant properties of carrot juice stabilised by high-intensity pulsed electric field or heat treatments. J Sci Food Agric 89:2363–2642
Rodríguez-Roque MJ, de Ancos B, Sánchez-Moreno C, Cano MP, Elez-Martínez P, Martín-Belloso O (2015) Impact of food matrix and processing on the in vitro bioaccessibility of vitamin C, phenolic compounds, and hydrophilic antioxidant activity from fruit juice-based beverages. J Funct Foods 14:33–43
Rodríguez-Roque MJ, de Ancos B, Sánchez-Vega R, Sánchez-Moreno C, Cano MP, Elez-Martínez P, Martín-Belloso O (2016) Food matrix processing influence on carotenoid bioaccessibility and lipophilic antioxidant activity of fruit-based beverages. Food and Function 7:380–389
Salvia-Trujillo L, Morales-de la Peña M, Rojas-Graü A, Martín-Belloso O (2011) Changes in water soluble vitamins and antioxidant capacity of fruit juice-milk beverages as affected by high-intensity pulsed electric field (HIPEF) or heat during chilled storage. J Agric Food Chem 59:10034–10043
Sánchez-Moreno C, Plaza L, Elez-Martínez P, De Ancos B, Martín-Belloso O, Cano MP (2005) Impact of high pressure and pulsed electric fields on bioactive compounds and antioxidant activity of orange juice in comparison with traditional thermal processing. J Agric Food Chem 53:4403–4409
Sánchez-Vega R, Elez-Martínez P, Martín-Belloso O (2015) Influence of high-intensity pulsed electric field processing parameters on antioxidant compounds of broccoli juice. Innov Food Sci Emerg Technol 29:70–77
Schilling S, Alber T, Toepfl S, Neidhart S, Knorr D, Schieber A, Carle R (2007) Effects of pulsed electric field treatment of apple mash on juice yield and quality attributes of apple juices. Innov Food Sci Emerg 8:127–134
Schilling S, Toepfl S, Ludwig M, Dietrich H, Knorr D, Neidhart S, Schieber A, Carle R (2008) Comparative study of juice production by pulsed electric field treatment and enzymatic maceration of apple mash. Eur Food Res Technol 226:1389–1398
Shohael AM, Ali MB, Yu KW, Hahn EJ, Islam R, Paek KY (2006) Effect of light on oxidative stress, secondary metabolites and induction of antioxidant enzymes in Eleutherococcus senticosus somatic embryos in bioreactor. Process Biochem 41:1179–1185
Soliva-Fortuny R, Balasa A, Knorr D, Martín-Belloso O (2009) Effects of pulsed electric fields on bioactive compounds in foods: a review. Trends Food Sci Tech 20:544–556
Sun B, Sato M, Clements JS (2000) Oxidative processes ocurring when pulsed high voltage discharges degrade phenol in aqueous solution. Environ Sci Technol 34:509–513
Taiwo KA, Angersbach A, Ade-Omowaye BIO, Knorr D (2001) Effects of pretreatments on the diffusion kinetics and some quality parameters of osmotically dehydrated apple slices. J Agric Food Chem 49:2804–2811
Taiwo KA, Angersbach A, Knorr D (2003) Effects of pulsed electric field on quality factors and mass transfer during osmotic dehydration of apples. J Food Process Eng 26:31–48
Teissié J, Golzio M, Rols MP (2005) Mechanisms of cell membrane electropermeabilization: a minireview or our present (lack of?) knowledge. Biochem Biophys Acta 1724:270–280
Torregrosa F, Esteve MJ, Frígola A, Cortés C (2006) Ascorbic acid stability during refrigerated storage of orange-carrot juice treated by high pulsed electric field and comparison with pasteurized juice. J Food Eng 73:339–345
Turk MF, Baron A, Vorobiev E (2010) Effect of pulsed electric fields treatment and mash size on extraction and composition of apple juices. J Agric Food Chem 58:9611–9616
Turk MF, Vorobiev E, Baron A (2012a) Improving apple juice expression and quality by pulsed electric field on an industrial scale. Food Sci Technol-LEB 49:245–250
Turk MF, Billaud C, Vorobiev E, Baron A (2012b) Continuous pulsed electric field treatment of French cider apple and juice expression on the pilot scale belt press. Innov Food Sci Emerg 14:61–69
Vallverdú-Queralt A, Oms-Oliu G, Odriozola-Serrano I, Lamuela-Raventós RM, Martín-Belloso O, Elez-Martínez P (2012a) Effects of pulsed electric fields on the bioactive compound content and antioxidant capacity of tomato fruit. J Agric Food Chem 60:3126–3134
Vallverdú-Queralt A, Odriozola-Serrano I, Oms-Oliu G, Lamuela-Raventós RM, Elez-Martínez P, Martín-Belloso O (2012b) Changes in the polyphenol profile of tomato juices processed by pulsed electric fields. J Agric Food Chem 60:9667–9672
Vallverdú-Queralt A, Oms-Oliu G, Odriozola-Serrano I, Lamuela-Raventós RM, Martín-Belloso O, Elez-Martínez P (2013a) Metabolite profiling of phenolic and carotenoid contents in tomatoes after moderate-intensity pulsed electric field treatments. Food Chem 136:199–205
Vallverdú-Queralt A, Odriozola-Serrano I, Oms-Oliu G, Lamuela-Raventós RM, Elez-Martínez P, Martín-Belloso O (2013b) Impact of high-intensity pulsed electric fields on carotenoids profile of tomato juice made of moderate-intensity pulsed electric field-treated tomatoes. Food Chem 141(3):3131–3138
Vervoort L, Van der Plancken I, Grauwet T, Timmermans RA, Mastwijk H, Matser AM, Hendrickx ME, Van Loey A (2011) Comparing equivalent thermal, high pressure and pulsed electric field processes for mild pasteurization of orange juice. Part II: impact on specific chemical and biochemical quality parameters. Innov Food Sci Emerg Technol 12:466–477
Weaver JC (2000) Electroporation of cells and tissues. IEEE Trans Plasma Sci 28:24–33
Xiang B, Sundararajan S, Solval KM, Espinoza-Rodezno L, Aryana K, Sathivel S (2014) Effects of pulsed electric field on physicochemical properties and microbial inactivation of carrot juice. J Food Process Preserv 38:1556–1564
Zeng X, Han Z, Zi Z (2010) Effects of pulsed electric field treatments on quality of peanut oil. Food Control 21:611–614
Zhang S, Yang R, Hua X, Zhang W, Zhang Z (2011) Influence of pulsed electric field treatments on the volatile compounds of milk in comparison with pasteurized processing. J Food Sci 76(1):C127–C132
Zhang Y, Liao XJ, Ni YY, Wu JH, Hu XS, Wang ZF, Chen F (2007) Kinetic analysis of the degradations and its color change of cyaniding-3-glucoside exposed to pulsed electric field. Eur Food Res Technol 224:597–603
Zhang Z-H, Zeng X-A, Brennan CS, Brennan M, Han Z, Xiong X-Y (2015) Effects of pulsed electric fields (PEF) on vitamin C and its antioxidant properties. Int J Mol Sci 16:24159–24173
Zulueta A, Barba F, Esteve MJ, Frígola A (2010) Effects on the carotenoid pattern and vitamin A of a pulsed electric field-treated orange juice-milk beverage and behavior during storage. Eur Food Res Technol 231:525–534
Zulueta A, Barba FJ, Esteve MJ, Frígola A (2013) Changes in quality and nutritional parameters during refrigerated storage of orange juice-milk beverage treated by equivalent thermal and non-thermal processes for mild pasteurization. Food Bioprocess Technol 6(8):2018–2030
Zulueta A, Esteve MJ, Frasquet I, Frigola A (2007) Fatty acid profile changes during orange juice-milk beverages processing by high-pulsed electric field. Eur J Lipid Sci Technol 109:25–31
Acknowledgements
The authors would like to thank the support of the Spanish Institute of Agricultural and Food Research and Technology (INIA) (project RTA2010-00079-C02-02) and the Spanish Ministry of Economy and Competitiveness (project AGL2013-44851-R).
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Elez-Martínez, P., Odriozola-Serrano, I., Oms-Oliu, G. et al. Effects of Pulsed Electric Fields Processing Strategies on Health-Related Compounds of Plant-Based Foods. Food Eng Rev 9, 213–225 (2017). https://doi.org/10.1007/s12393-017-9162-x
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DOI: https://doi.org/10.1007/s12393-017-9162-x
