Isolation of a cDNA that encodes a novel granulocyte N-formyl peptide receptor
Abstract
A cDNA of 1650 base pairs was isolated by screening an HL-60 granulocyte library with an N-formyl peptide receptor (NFPR) cDNA probe under low stringency conditions. The cDNA encodes a protein of 351 amino acids tentatively named FPR2, with a calculated molecular weight of 39 kDa. Sequence analysis revealed that FPR2 is 69% identical in sequence to the human NFPR and shares extensive homology to several other chemoattractant receptors. FPR2 expressed in transfected cells mediated formyl peptide-stimulated calcium mobilization at micromolar concentrations of ligand. FPR2 messenger is detected in granulocytic HL-60 cells, but not in undifferentiated HL-60 cells. These findings suggest that FPR2 is a novel receptor for formyl peptide ligand and a new member of the chemoattractant receptor gene family.
- Publication:
-
Biochemical and Biophysical Research Communications
- Pub Date:
- 1992
- DOI:
- Bibcode:
- 1992BBRC..184..582Y
- Keywords:
-
- NFPR;
- N-formyl peptide receptor;
- G protein;
- guanine nucleotide regulatory protein;
- FLPEP;
- N-formyl-Nle-Leu-Phe-Nle-Lys-fluorescein;
- fMLP;
- fMet-Leu-Phe