A hydrogen-producing, hydrogenase-free mutant strain of Nostoc punctiforme ATCC 29133
Abstract
The hupL gene, encoding the uptake hydrogenase large subunit, in Nostoc sp. strain ATCC 29133, a strain lacking a bidirectional hydrogenase, was inactivated by insertional mutagenesis. Recombinant strains were isolated and analysed, and one hupL ‑ strain, NHM5, was selected for further study. Cultures of NHM5 were grown under nitrogen-fixing conditions and H 2 evolution under air was observed using an H 2 electrode.
- Publication:
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International Journal of Hydrogen Energy
- Pub Date:
- January 2002
- DOI:
- Bibcode:
- 2002IJHE...27.1291L
- Keywords:
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- Nostoc;
- Uptake hydrogenase mutant;
- Nitrogenase;
- Hydrogen evolution