Possibilities and limitations of label-free detection of DNA hybridization with field-effect-based devices
Abstract
A critical evaluation of the possibilities and limitations of the label-free detection of deoxyribonucleic acid (DNA) hybridization by means of field-effect-based devices is discussed. A new DNA-detection method is introduced, which utilizes an ion-sensitive field-effect device as transducer. The upon the DNA hybridization induced redistribution of the ion concentration within the intermolecular spaces and/or the alteration of the ion sensitivity of the device is proposed as detection mechanism. The theoretical calculations predict a substantial change in the average ion concentration within the intermolecular spaces induced upon hybridization that are enough to obtain a detectable sensor signal.
- Publication:
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Sensors and Actuators B: Chemical
- Pub Date:
- January 2005
- DOI:
- Bibcode:
- 2005SeAcB.111..470P
- Keywords:
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- DNA;
- Label-free detection;
- Field-effect device;
- Ion-sensitive sensor