doi: 10.1093/nar/13.7.2399.
The synthesis of oligonucleotides containing an aliphatic amino group at the 5' terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis
- PMID: 4000959
- PMCID: PMC341163
- DOI: 10.1093/nar/13.7.2399
Item in Clipboard
The synthesis of oligonucleotides containing an aliphatic amino group at the 5' terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis
Nucleic Acids Res.
.
Free PMC article
Abstract
A rapid and versatile method has been developed for the synthesis of oligonucleotides which contain an aliphatic amino group at their 5' terminus. This amino group reacts specifically with a variety of electrophiles, thereby allowing other chemical species to be attached to the oligonucleotide. This chemistry has been utilized to synthesize several fluorescent derivatives of an oligonucleotide primer used in DNA sequence analysis by the dideoxy (enzymatic) method. The modified primers are highly fluorescent and retain their ability to specifically prime DNA synthesis. The use of these fluorescent primers in DNA sequence analysis will enable DNA sequence analysis to be automated.
Similar articles
-
Non-radioactive automated sequencing of oligonucleotides by chemical degradation.Nucleic Acids Res. 1988 Mar 25;16(5):2203-6. doi: 10.1093/nar/16.5.2203. Nucleic Acids Res. 1988. PMID: 3357772 Free PMC article.
-
Specific-primer-directed DNA sequencing using automated fluorescence detection.Nucleic Acids Res. 1989 Aug 11;17(15):6087-102. doi: 10.1093/nar/17.15.6087. Nucleic Acids Res. 1989. PMID: 2771640 Free PMC article.
-
Synthesis and primer properties of oligonucleotides containing 3'-deoxypsicothymidine units, labeled with fluorescein at the 1'-position.Bioconjug Chem. 1994 Nov-Dec;5(6):501-3. doi: 10.1021/bc00030a003. Bioconjug Chem. 1994. PMID: 7873654
-
High-performance liquid chromatography of DNA.J Chromatogr. 1984 Dec 7;336(1):3-14. doi: 10.1016/s0378-4347(00)85125-7. J Chromatogr. 1984. PMID: 6098596 Review. No abstract available.
-
Automated synthesis and sequence analysis of biological macromolecules.Anal Chem. 1988 Mar 15;60(6):381A-390A. doi: 10.1021/ac00157a001. Anal Chem. 1988. PMID: 3288001 Review. No abstract available.
Cited by
-
Hybridization histochemistry.Experientia. 1987 Jul 15;43(7):741-50. doi: 10.1007/BF01945351. Experientia. 1987. PMID: 3297764 Review.
-
The synthesis of protected 5'-amino-2',5'-dideoxyribonucleoside-3'-O-phosphoramidites; applications of 5'-amino-oligodeoxyribonucleotides.Nucleic Acids Res. 1987 Aug 11;15(15):6181-96. doi: 10.1093/nar/15.15.6181. Nucleic Acids Res. 1987. PMID: 3627984 Free PMC article.
-
Next-Generation Sequencing (NGS) and Third-Generation Sequencing (TGS) for the Diagnosis of Thalassemia.Diagnostics (Basel). 2023 Jan 19;13(3):373. doi: 10.3390/diagnostics13030373. Diagnostics (Basel). 2023. PMID: 36766477 Free PMC article. Review.
-
Preparation of oligodeoxynucleotide-alkaline phosphatase conjugates and their use as hybridization probes.Nucleic Acids Res. 1986 Aug 11;14(15):6115-28. doi: 10.1093/nar/14.15.6115. Nucleic Acids Res. 1986. PMID: 3748805 Free PMC article.
-
Efficient methods for attaching non-radioactive labels to the 5' ends of synthetic oligodeoxyribonucleotides.Nucleic Acids Res. 1986 Aug 11;14(15):6227-45. doi: 10.1093/nar/14.15.6227. Nucleic Acids Res. 1986. PMID: 3748808 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
