Hi EGAPx developers,
I am currently using EGAPx to annotate a water lily genome (Nymphaea colorata).
For the annotation, I have provided RNA-seq data as transcript evidence and specified the corresponding taxonomic ID. However, EGAPx stops during the analysis with the following error:
ERROR: Proteins are not found for tax id 4418
This organism is not supported by current NCBI protein database
You can specify proteins manually using 'proteins' parameter
The RNA-seq data were successfully provided, and the workflow starts normally before reaching this step.
I would like to ask:
Is Nymphaea / Nymphaea colorata currently supported by EGAPx?
Can this problem be solved by providing a custom protein FASTA file?
If so, what is the recommended way to specify the proteins parameter?
Can proteins from Nymphaea colorata or closely related species be used as protein evidence?
Is there any recommended approach for annotating species whose taxonomic group is not currently represented in the default EGAPx protein database?
Any advice on how to proceed with EGAPx annotation for Nymphaea colorata would be greatly appreciated.
Thank you for your help.
Hi EGAPx developers,
I am currently using EGAPx to annotate a water lily genome (Nymphaea colorata).
For the annotation, I have provided RNA-seq data as transcript evidence and specified the corresponding taxonomic ID. However, EGAPx stops during the analysis with the following error:
ERROR: Proteins are not found for tax id 4418
This organism is not supported by current NCBI protein database
You can specify proteins manually using 'proteins' parameter
The RNA-seq data were successfully provided, and the workflow starts normally before reaching this step.
I would like to ask:
Is Nymphaea / Nymphaea colorata currently supported by EGAPx?
Can this problem be solved by providing a custom protein FASTA file?
If so, what is the recommended way to specify the proteins parameter?
Can proteins from Nymphaea colorata or closely related species be used as protein evidence?
Is there any recommended approach for annotating species whose taxonomic group is not currently represented in the default EGAPx protein database?
Any advice on how to proceed with EGAPx annotation for Nymphaea colorata would be greatly appreciated.
Thank you for your help.