We designed a widely applicable method to identify signal-activated transcription factors (TFs) without any prior knowledge of their properties. To establish proof of concept for BC-STAR-PROM, we applied it to the identification of TFs induced by drugs affecting actin and tubulin cytoskeleton dynamics.

This repository is associated to the original publication:

Unbiased identification of signal-activated transcription factors by barcoded synthetic tandem repeat promoter screening (BC-STAR-PROM)

by Pauline Gosselin, Gianpaolo Rando, Fabienne Fleury-Olela, and Ueli Schibler University of Geneva (CH)

Genes Dev. August 15, 2016 30: 1895-1907; doi:10.1101/gad.284828.116

The repository contains: R script fold changes tables Original step-by-step protocol Final publication (open access)

Both script and protocol were written for personal use. For more information contact: Gianpaolo Rando gianpaolo_DOT_rando_AT_swissdecode_DOT_com

The plasmid library should be soon available on Addgene.