Synthetic Biology

Bacterial whole-cell biosensors illuminate spatially variable sialic acid availability within the inflamed mammalian gut
David Carreno, Clare M Robinson, Raphaella Jackson, Peirong Li, Vanessa Nunes, Susan A Palma-Duran, Emma Nye, James I MacRae, David T Riglar

bioRxiv 2024.10.23.619804; doi: https://doi.org/10.1101/2024.10.23.619804 New Results

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Combinatorial optimization of protein systems in synthetic cells
Marijn van den Brink, Nico J Claassens, Christophe Danelon

bioRxiv 2026.02.25.707944; doi: https://doi.org/10.64898/2026.02.25.707944 New Results

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Tunable Low-Rate Genomic Recombination with Cre-lox in Escherichia coli: A Versatile Tool for Anoxic Environmental Biosensing and Synthetic Biology
Elisa Garabello, Hyun Yoon, Matthew C. Reid, Andrea Giometto

bioRxiv 2024.10.02.616356; doi: https://doi.org/10.1101/2024.10.02.616356 New Results

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CyanOperon: an operon building expansion for the CyanoGate MoClo toolkit
Michael J. Astbury, Alejandra A. Schiavon Osorio, Angelo J. Victoria, Alistair J. McCormick

bioRxiv 2026.02.24.707249; doi: https://doi.org/10.64898/2026.02.24.707249 New Results

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A portable orthogonal replication system enables continuous gene evolution near the biological speed limit
Rongzhen Tian, Fabian B. H. Rehm, Matthew Kenneth, Kiarash Jamali, Petar S. Zhotev, Kim C. Liu, Jason W. Chin

bioRxiv 2026.02.20.706958; doi: https://doi.org/10.64898/2026.02.20.706958 New Results

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TargetMITO: A rule-based model for generating highly functional synthetic mitochondrial targeting sequence in yeast
Kewin Gombeau, Raymond Wan, Joshua S. James, Déborah Tribouillard-Tanvier, Yizhi Cai

bioRxiv 2026.02.22.707306; doi: https://doi.org/10.64898/2026.02.22.707306 New Results

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Programmable nanobody circuits for cell selection
Nathan B. Wang, Albert Blanch-Asensio, Hannah Cevasco, Deon S. Ploessl, Derin R. Gumustop, Mary E. Ehmann, Maria F. Castellanos, Francisco J. Sánchez-Rivera, Timothy M. O’Shea, Kate E. Galloway

bioRxiv 2026.02.22.707289; doi: https://doi.org/10.64898/2026.02.22.707289 New Results

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Heterologous Rieske non-heme iron monooxygenases enable efficient microbial conversion of lignin guaiacol to adipic acid
John F.C. Steele, Charles B. Wackwitz, Gary Walker, Krishnan T. Selvy, Stephen Wallace

bioRxiv 2026.02.23.707458; doi: https://doi.org/10.64898/2026.02.23.707458 New Results

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Nucleotide-level chemical reaction network modeling enables quantitative prediction of reconstituted cell-free expression system
Zoila Jurado, Ayush Pandey, Richard M. Murray

bioRxiv 2026.02.22.707325; doi: https://doi.org/10.64898/2026.02.22.707325 New Results

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Efficient cell-free evolution of RNA polymerases by droplet microfluidics
Naohiro Terasaka, Taro Furubayashi, Kenya Tajima, Hiroyuki Noji

bioRxiv 2026.02.23.707346; doi: https://doi.org/10.64898/2026.02.23.707346 New Results

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Synthetic Biology

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