Questions tagged [biotechnology]
Biotechnology is a field of biology which utilizes biological systems like microorganisms to produce valuable products, such as biofuels, drugs, and vaccines.
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What is the best method for sequencing mRNA from exosomes?
Say you wanted to compare extracellular vesicle (EV) mRNA between two cell lines and had isolated extracellular vesicles and extracted total RNA from them.
What then would be the best sequencing ...
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Recombinant insulin production and cyanogen bromide action
For recombinant insulin production from E. coli (according to Eli Lilly), cyanogen bromide is used to cleave residues in residual $\beta$-galactosidase. It is well established that cyanogen bromide ...
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RNAi for gene silencing
According to NCERT Biology Class XII Biology textbook, pg. 180, it is given as follows
Several nematodes parasitise a wide variety of
plants and animals including human beings. A nematode Meloidegyne
...
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“How can I improve survival and attachment of SIM-A9 microglial cells after thawing from cryopreservation?
I am working with SIM-A9 microglial cells in vitro and I have been struggling with issues after cryopreservation. When I thaw and re-plate the cells, I consistently face problems with attachment and ...
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How "close" are genome-edited "de-extinct" animals to their extinct counterparts?
I'm not a biologist, so I apologize if this question is a bit vague. But since it's currently a highly discussed topic in the media, I’d really like to understand it better — and I imagine others ...
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Restriction endonucleases isolated from H. influenzae
I was going through this site and it said
Hind III is the third endonuclease of four isolated from Haemophilus influenza, serotype d.
HindI, HindII and HindIII being three out of the four, what is ...
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Could EM fields be able to prevent ice crystal formation within the cells of a cryopreserved human?
It was just announced that Hasheem Al-Ghali, a molecular biologist, science communicator and futurist and a A.I. scientist and doctor are creating a company called TimeShift, which is a company ...
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What happens if we use Pvu2 to insert a gene in the rop of pBR322 [closed]
My book shows that pBR322 has a Pvu II site in the rop, I know that rop codes for proteins required for replication of the plasmid, so what happens if we use the Pvu II Restriction Enzyme and insert a ...
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Can saltwater bacteria grow in petri-dish using the same agar nutrient media?
I am planning on growing bacteria from a seawater sample to do a research project on the relationship between pH (lowered using sulfuric acid) and the bacterial concentration of the water sample (...
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Oxygen requirement of Eschericia coli
What is a good DO% range for E. coli cultivation (BL21(DE3), recombinant protein expression). 30%? At what point is a culture oxygen-limited, or 'highly aerated'? If DO% is relative to reactor ...
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What is the minimum Ct value that you would consider indicates no gene expression?
I am doing relative quantification to compare gene expression between a control with several mutant backgrounds using qPCR.
Looking online I find several different sources suggesting different values ...
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Is there potential to modify GM crops to inhibit their reproduction with non-GM crops?
I've been reading on terminator gene sequences and was wondering whether the same technology could be applied to GM crops to prevent transgene flow. Turns out Monsanto had developed the technology but ...
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Does blunt end have a hydrogen bond?
I am an undergraduate student of biology.
I saw a lecturer online, who said that sticky ends makes phosphodiester and hydrogen bonds cut. (also here).
But why blunt end just makes phospodiester bonds ...
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Which was the first restriction endonuclease to be isolated?
I kind of have confusion between the first restriction endonuclease to be isolated and discovered.
In class XII NCERT, it says that,
"In the year 1963, the two enzymes responsible for restricting ...
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Recent developments in the diagnosis of Parkinson's Disease
I was reading a research paper titled - "Discovery of Volatile Biomarkers of Parkinson’s Disease from Sebum" by Drupad K. Trivedi. I am Providing a short summary of it.
Discovery of ...
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